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Hypersil BDS C18 250mm x 4.6mm x 5um

The Thermo Scientific™ Hypersil™ BDS C18 HPLC Column (250 mm × 4.6 mm, 5 µm) is a high-performance reversed-phase analytical HPLC column designed for accurate, reliable, and reproducible chromatographic separations. Built on the renowned Hypersil silica backbone with base-deactivated silica (BDS) technology, this column minimizes residual silanol activity, resulting in improved peak symmetry, reduced tailing for basic compounds, and excellent chromatographic performance across a wide range of analytical applications.

Engineered for quality control (QC), quality assurance (QA), pharmaceutical, environmental, food, biotechnology, and research laboratories, the Hypersil BDS C18 column provides excellent reproducibility, durability, and consistent separation efficiency. The 5 µm fully porous spherical silica particles combined with a 250 mm column length deliver high resolving power for complex sample analysis while maintaining dependable performance under routine laboratory conditions.

SKU: 28105-254633 Category: Brand:

Specifications

Parameter Specification
Product Type Reversed-Phase HPLC Column
Brand Thermo Scientific™
Product Line Hypersil™ BDS
Stationary Phase C18
Column Format Analytical Column
Column Dimensions 250 × 4.6 mm
Column Length 250 mm
Internal Diameter 4.6 mm
Particle Size 5 µm
Packing Material Spherical Fully Porous Base-Deactivated Silica
End-Capped Yes
Carbon Load 11%
Pore Size 130 Å
Surface Area 170 m²/g
pH Range 2–8
Maximum Pressure 400 bar (5800 psi)
Maximum Temperature 60°C
USP Classification L1

Features

  • Thermo Scientific™ Hypersil™ BDS C18 analytical HPLC column
  • Column dimensions: 250 mm × 4.6 mm
  • 5 µm spherical fully porous particles
  • Reversed-phase C18 stationary phase
  • Base-Deactivated Silica (BDS) technology
  • End-capped stationary phase
  • Reduced residual silanol activity
  • Excellent peak symmetry for basic compounds
  • High chromatographic reproducibility
  • Robust and economical column for routine analysis
  • 130 Å pore size
  • 11% carbon loading
  • Surface area of 170 m²/g
  • pH operating range: 2–8
  • Maximum operating pressure: 400 bar (5800 psi)
  • Maximum operating temperature: 60°C
  • USP Classification: L1
  • Suitable for QC, QA, pharmaceutical, food, environmental, and research laboratories

Benefits

  • Excellent Peak Shape: Base-deactivated silica minimizes silanol interactions, reducing peak tailing and improving separation of basic analytes.
  • High Resolution Separations: The 250 mm column length provides excellent resolving power for complex analytical samples.
  • Outstanding Reproducibility: Manufactured using Thermo Scientific’s proven Hypersil silica technology for consistent batch-to-batch performance.
  • Versatile Analytical Performance: Suitable for pharmaceutical, food, environmental, chemical, and biotechnology HPLC applications.
  • Reliable Routine Operation: Designed as an economical, general-purpose HPLC column for daily laboratory use.
  • Wide Method Compatibility: Compatible with a broad range of reversed-phase HPLC methods across pH 2–8.
  • Long Service Life: Durable end-capped silica packing helps improve column stability and operational lifetime.
  • Improved Method Validation: High reproducibility supports reliable QC, QA, and regulatory analytical workflows.
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