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250×4.6mm 5um GOLD C4

The Thermo Scientific™ Hypersil GOLD™ C4 HPLC Column (250 × 4.6 mm, 5 µm) is a premium reversed-phase analytical HPLC column engineered for the separation of hydrophobic proteins, peptides, biomolecules, and other strongly retained analytes. Featuring a C4 bonded stationary phase with lower hydrophobicity than conventional C18 and C8 columns, it delivers excellent selectivity, faster elution, superior peak symmetry, and high chromatographic efficiency.

Manufactured using high-purity spherical fully porous silica, the Hypersil GOLD™ C4 column ensures exceptional reproducibility, sensitivity, and long-term reliability. Its 250 mm length, 4.6 mm internal diameter, and 5 µm particle size provide high-resolution separations, making it an excellent choice for pharmaceutical quality control, biopharmaceutical analysis, proteomics, peptide purification, biotechnology, food testing, and research laboratories.

SKU: 25505-254630 Category: Brand:

Specifications

Parameter Specification
Product Type Analytical HPLC Column
Brand Thermo Scientific™
Product Series Hypersil GOLD™
Stationary Phase C4
Chromatography Type Reversed Phase
Column Dimensions 250 × 4.6 mm
Particle Size 5 µm
Particle Type Fully Porous
Packing Material Spherical Ultrapure Silica
Endcapped Yes
USP Classification L26
Carbon Load 5%
Pore Size 175 Å
Surface Area 220 m²/g
pH Range 2–8
Maximum Pressure 400 bar (5800 psi)
Maximum Temperature 60°C
Column Format Analytical Column
Application Protein, Peptide & Hydrophobic Compound Analysis

Features

  • Analytical reversed-phase HPLC column
  • Thermo Scientific™ Hypersil GOLD™ C4 chemistry
  • Column dimensions: 250 × 4.6 mm
  • Particle size: 5 µm
  • C4 bonded stationary phase with lower hydrophobicity
  • Fully porous spherical ultrapure silica particles
  • Endcapped stationary phase for improved peak symmetry
  • Excellent separation of hydrophobic proteins and peptides
  • Faster elution compared to C18 and C8 columns
  • High chromatographic efficiency and sensitivity
  • 175 Å pore size for biomolecule analysis
  • 5% carbon load
  • pH operating range: 2–8
  • Maximum operating pressure: 400 bar (5800 psi)
  • Temperature limit: 60°C
  • USP Classification: L26
  • Excellent batch-to-batch reproducibility
  • Compatible with standard HPLC systems
  • Ideal for pharmaceutical, biotechnology, and research laboratories

Benefits

  • Optimized for Hydrophobic Biomolecules: The C4 stationary phase provides excellent retention and separation of hydrophobic proteins, peptides, and biomolecules with reduced retention compared to C18 columns.
  • Superior Peak Symmetry: Endcapped silica chemistry minimizes secondary interactions, producing sharp, symmetrical peaks and improved analytical accuracy.
  • High Resolution & Sensitivity: Fully porous ultrapure silica particles deliver excellent column efficiency, narrow peaks, and enhanced detection sensitivity.
  • Reliable Reproducibility: Advanced Hypersil GOLD™ chemistry ensures consistent chromatographic performance from column to column.
  • Ideal for Pharmaceutical Analysis: Suitable for QC laboratories, method development, impurity profiling, peptide mapping, and protein analysis.
  • Robust Chemical Stability: Operates reliably across a pH range of 2–8, making it suitable for a wide variety of reversed-phase HPLC methods.
  • Long Column Life: High-purity silica and durable bonded phase provide excellent performance over extended routine use.

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