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250×4.6mm 5um GOLD Silica

The Thermo Scientific™ Hypersil GOLD™ Silica HPLC Column (250 × 4.6 mm, 5 µm) is a premium analytical chromatography column designed for normal-phase and HILIC (Hydrophilic Interaction Liquid Chromatography) separations. Packed with ultrapure, base-deactivated, fully porous silica, the column delivers excellent peak symmetry, high efficiency, and outstanding reproducibility for separating nonpolar and moderately polar organic compounds.

Manufactured using Thermo Scientific’s advanced Hypersil GOLD technology, the column provides enhanced sensitivity, improved resolution, and exceptional batch-to-batch consistency. The 250 mm column length offers high resolving power for complex analytical separations, while the 5 µm particle size ensures reliable performance for routine HPLC applications.

SKU: 25105-254630 Category: Brand:

Specifications

Parameter Specification
Product Type Analytical HPLC Column
Brand Thermo Scientific™
Product Series Hypersil GOLD™
Stationary Phase Silica (USP L3)
Chromatography Type Normal Phase / HILIC
Column Dimensions 250 × 4.6 mm
Particle Size 5 µm
Particle Type Fully Porous
Packing Material Ultrapure Base-Deactivated Silica
Surface Area 220 m²/g
Pore Size 175 Å
pH Range 2–8
Maximum Pressure 400 bar (5800 psi)
Maximum Temperature 60°C
Application Analytical HPLC

Features

  • Analytical HPLC column for normal-phase and HILIC chromatography
  • Column dimensions: 250 × 4.6 mm
  • Particle size: 5 µm
  • Ultrapure, base-deactivated silica stationary phase
  • Fully porous spherical silica particles
  • Excellent peak symmetry and narrow peak widths
  • High column efficiency and sensitivity
  • Improved chromatographic resolution
  • Exceptional batch-to-batch reproducibility
  • Optimized for separating nonpolar and moderately polar compounds
  • Stable operating pH range of 2–8
  • Surface area of 220 m²/g
  • Pore size of 175 Å
  • Maximum operating pressure of 400 bar (5800 psi)
  • Maximum operating temperature of 60°C
  • Compatible with standard analytical HPLC systems
  • USP Classification: L3

Benefits

  • Superior Chromatographic Performance: Produces excellent peak shapes and improved sensitivity for accurate analytical results.
  • High Resolution Separations: The 250 mm column length provides enhanced separation efficiency for complex sample mixtures.
  • Outstanding Reproducibility: High-purity silica and advanced manufacturing deliver consistent results from column to column.
  • Optimized for Normal-Phase Chromatography: Ideal for separating nonpolar and moderately polar analytes with reliable performance.
  • Enhanced Laboratory Productivity: Narrow peak widths and excellent efficiency improve analytical throughput.
  • Long Column Life: High-quality silica packing provides durable performance under routine laboratory operating conditions.
  • Wide Analytical Applications: Suitable for pharmaceutical, chemical, food, environmental, forensic, and research laboratories.
  • Reliable Method Development: Consistent selectivity and reproducibility support method validation and quality control applications.

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