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ACCLAIM 120 C18 5UM 4.6X250MM

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ACCLAIM 120 C18 5UM 4.6X250MM
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ACCLAIM 120 C18 5UM 4.6X250MM

The Thermo Scientific™ Acclaim™ 120 C18 HPLC Column (5 µm, 4.6 × 250 mm) is a high-performance reversed-phase analytical chromatography column designed to deliver exceptional resolution, reproducibility, and peak symmetry across a wide range of HPLC and LC/MS applications. Built using ultrapure fully porous silica with advanced bonding and end-capping technology, the column minimizes secondary interactions and peak tailing while ensuring consistent chromatographic performance.

Designed for pharmaceutical, biopharmaceutical, environmental, food, chemical, and research laboratories, the Acclaim™ 120 C18 provides reliable separation for both routine and complex analytical methods. Its 120 Å pore size, 18% carbon loading, and high surface area enable excellent retention and resolution for a broad spectrum of analytes. The column is fully compatible with LC/MS applications due to its extremely low column bleed, making it an ideal choice for high-sensitivity analyses.

SKU: 59149 Category: Brand:

Technical Specifications:

Parameter Specification
Product Type Reversed-Phase Analytical HPLC Column
Brand Thermo Scientific™
Product Series Acclaim™ 120 C18
Stationary Phase C18 (USP L1)
Column Dimensions 4.6 × 250 mm
Particle Size 5 µm
Pore Size 120 Å
Packing Material Spherical Fully Porous Ultrapure Silica
Carbon Load 18%
Surface Area 300 m²/g
pH Range 2–8
Maximum Pressure 4500 psi (310 bar)
Maximum Temperature 60°C
Column Format Analytical Column
LC/MS Compatibility Yes (Extremely Low Bleed)
Primary Applications HPLC, LC/MS, Pharmaceutical, Environmental & Research Analysis

Features

  • High-performance reversed-phase C18 analytical HPLC column
  • Column dimensions: 4.6 × 250 mm
  • Particle size: 5 µm
  • Pore size: 120 Å
  • Ultrapure fully porous silica packing material
  • Advanced bonding and end-capping technology
  • High-efficiency chromatographic separation
  • Excellent peak symmetry with minimal peak tailing
  • Extremely low column bleed for LC/MS compatibility
  • High surface coverage for reproducible performance
  • Stable operation across pH 2–8
  • Maximum operating pressure: 4500 psi (310 bar)
  • Carbon loading: 18%
  • Surface area: 300 m²/g
  • Maximum operating temperature: 60°C
  • USP L1 stationary phase
  • Suitable for pharmaceutical, environmental, food, and research laboratories

Benefits

  • Superior Chromatographic Resolution: Delivers excellent separation efficiency for a wide variety of compounds in analytical HPLC applications.
  • Excellent Peak Shape: Advanced end-capping technology minimizes secondary interactions, resulting in symmetrical peaks and reduced tailing.
  • High Reproducibility: Provides consistent column-to-column performance for reliable analytical results.
  • LC/MS Compatible: Extremely low bleed characteristics make the column suitable for high-sensitivity LC/MS applications.
  • Broad Application Compatibility: Ideal for pharmaceutical analysis, environmental testing, food safety, biotechnology, and research laboratories.
  • Stable Performance: Operates reliably over a pH range of 2–8 with excellent chemical stability.
  • Long Column Life: High-purity silica packing and robust bonding technology enhance durability and analytical performance.
  • Reliable Analytical Results: Supports accurate qualitative and quantitative analysis in quality control and research laboratories.

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