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BIOBASIC SEC 120 5UM 300X7.8MM PREP COLUMN

The Thermo Scientific™ BioBasic™ SEC 120 5 µm 300 × 7.8 mm Preparative HPLC Column is a high-performance Size Exclusion Chromatography (SEC) column designed for the efficient separation and purification of water-soluble biomolecules based on their molecular size and shape. Engineered with a 120 Å pore size and 5 µm spherical ultrapure silica particles bonded with a diol (OH) stationary phase, this preparative column delivers high separation efficiency, excellent reproducibility, and long operational life for laboratory and preparative chromatography applications.

The BioBasic SEC 120 Preparative Column is ideal for separating proteins, peptides, enzymes, antibodies, nucleic acids, and other biomolecules across a molecular weight range of approximately 700 to 120,000 Daltons. The size exclusion mechanism minimizes secondary interactions, ensuring gentle separation while preserving the native structure and biological activity of sensitive biomolecules.

SKU: 73405-307846A Category: Brand:

Specifications

Parameter Specification
Product Type Preparative Size Exclusion HPLC Column
Brand Thermo Scientific™
Product Line BioBasic™ PREP SEC 120
Chromatography Mode Size Exclusion Chromatography (SEC)
Column Dimensions 300 × 7.8 mm
Particle Size 5 µm
Pore Size 120 Å
Stationary Phase Diol (OH)
Packing Material Spherical, Fully Porous Ultrapure Silica
Molecular Weight Range Approximately 700–120,000 Da
Maximum Pressure 4300 psi (295 bar)
Operating pH Range 2–8
Maximum Temperature 60°C
USP Classification L59
Application Preparative Biomolecule Separation & Purification

Features

  • Thermo Scientific™ BioBasic™ PREP SEC 120 HPLC Column
  • Preparative Size Exclusion Chromatography (SEC) column
  • Column dimensions: 300 × 7.8 mm
  • Particle size: 5 µm
  • Pore size: 120 Å
  • Diol (OH) stationary phase
  • Spherical, fully porous ultrapure silica packing
  • High-efficiency biomolecule separation
  • Molecular weight separation based on size and shape
  • Suitable for proteins, peptides, enzymes, and biomolecules
  • Wide molecular weight separation range (700–120,000 Da)
  • Excellent sample cleanup capability
  • Long column life with high reproducibility
  • Simple method development using aqueous mobile phases
  • Maximum operating pressure: 4300 psi (295 bar)
  • pH operating range: 2–8
  • Maximum operating temperature: 60°C
  • Suitable for preparative chromatography applications

Benefits

  • High-Efficiency Size Separation: Provides accurate separation of biomolecules based solely on molecular size, reducing unwanted secondary interactions.
  • Excellent Biomolecule Recovery: The diol stationary phase helps preserve the biological activity of proteins, peptides, and other sensitive compounds during purification.
  • Broad Molecular Weight Range: Suitable for biomolecules ranging from approximately 700 to 120,000 Daltons, making it versatile for various life science applications.
  • Ideal for Preparative Purification: The 300 × 7.8 mm preparative format supports efficient purification and isolation of biomolecules.
  • Simple Method Development: SEC requires uncomplicated aqueous mobile phases, simplifying analytical and preparative workflows.
  • Long Operational Life: High-purity spherical silica particles provide consistent performance and extended column durability.
  • Excellent Sample Cleanup: Suitable for desalting, buffer exchange, aggregate removal, and purification before downstream analysis.
  • Reliable Reproducibility: Consistent packing material ensures repeatable chromatographic performance across multiple runs.

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