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HYPERSIL BDS C18 250 X 4MM 5 MICRON

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HYPERSIL BDS C18 250 X 4MM 5 MICRON

The Thermo Scientific™ Hypersil™ BDS C18 HPLC Column (250 × 4.0 mm, 5 µm) is a high-performance reversed-phase analytical HPLC column designed for reliable and reproducible chromatographic separations in pharmaceutical, environmental, food, chemical, clinical, and research laboratories. Built on the renowned Hypersil™ base-deactivated silica (BDS) technology, the column offers reduced residual silanol activity, resulting in improved peak symmetry, excellent reproducibility, and enhanced performance for a wide range of analytical applications.

Featuring a C18 stationary phase with 5 µm spherical fully porous particles, the column delivers excellent separation efficiency and robustness for routine quality control (QC), quality assurance (QA), and research laboratories. The 250 mm column length provides enhanced resolution for complex sample separations, while the 4.0 mm internal diameter offers an optimal balance between sensitivity and solvent consumption.

SKU: 28105-254030 Category: Brand:

Specifications

Parameter Specification
Product Type Reversed-Phase HPLC Column
Brand Thermo Scientific™
Product Line Hypersil™ BDS
Stationary Phase C18
Column Format Analytical Column
Column Dimensions 250 × 4.0 mm
Column Length 250 mm
Internal Diameter 4.0 mm
Particle Size 5 µm
Packing Material Spherical Fully Porous Base-Deactivated Silica
End-Capped Yes
USP Classification L1
Pore Size 130 Å
Carbon Load 11%
Surface Area 170 m²/g
Operating pH Range 2–8
Maximum Pressure 400 bar (5800 psi)
Maximum Temperature 60°C

Features

  • Thermo Scientific™ Hypersil™ BDS C18 HPLC Column
  • Column dimensions: 250 × 4.0 mm
  • Particle size: 5 µm
  • Reversed-phase analytical HPLC column
  • Base-deactivated silica (BDS) technology
  • C18 stationary phase
  • End-capped packing for improved peak symmetry
  • Spherical fully porous silica particles
  • USP L1 column classification
  • 130 Å pore size
  • 11% carbon loading
  • Surface area: 170 m²/g
  • Operating pH range: 2–8
  • Maximum operating pressure: 400 bar (5800 psi)
  • Maximum operating temperature: 60°C
  • Excellent batch-to-batch reproducibility
  • Suitable for routine QC/QA and research applications

Benefits

  • Excellent Chromatographic Performance: Base-deactivated silica minimizes residual silanol interactions, producing sharper peaks and improved chromatographic performance.
  • High Separation Efficiency: The 250 mm column length provides excellent resolution for complex mixtures and challenging analytical separations.
  • Improved Peak Symmetry: End-capped C18 chemistry helps reduce tailing, especially for basic analytes.
  • Reliable Reproducibility: Manufactured using Hypersil™ silica technology for consistent batch-to-batch performance and dependable analytical results.
  • Versatile Analytical Applications: Suitable for pharmaceutical analysis, environmental testing, food safety analysis, chemical testing, and research laboratories.
  • Wide Method Compatibility: Compatible with a broad range of reversed-phase HPLC methods operating between pH 2 and 8.
  • Long Service Life: Robust silica packing and high mechanical stability support extended column performance during routine laboratory use.
  • Optimized for QC & QA Laboratories: Designed as a reliable, economical general-purpose HPLC column for routine analytical workflows.

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