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HYPERSIL HPLC COLUMN 250 X 4.6MM 3UM BDS C18

The Thermo Scientific™ Hypersil™ BDS C18 HPLC Column (250 × 4.6 mm, 3 µm) is a premium reversed-phase analytical HPLC column engineered for high-resolution chromatographic separations in pharmaceutical, environmental, food, chemical, biotechnology, and research laboratories. Built on the renowned Hypersil base-deactivated silica (BDS) technology, this column delivers exceptional peak symmetry, reproducibility, and column longevity for routine and demanding analytical applications.

The Hypersil BDS C18 stationary phase features minimal residual silanol activity, reducing secondary interactions with basic compounds and improving peak shape. Its 3 µm spherical fully porous silica particles provide excellent separation efficiency, making the column ideal for complex sample analysis and high-performance HPLC methods.

SKU: 28103-254630 Category: Brand:

Specifications

Parameter Specification
Product Type Reversed-Phase HPLC Column
Brand Thermo Scientific™
Product Line Hypersil™ BDS
Stationary Phase C18
Column Dimensions 250 × 4.6 mm
Internal Diameter 4.6 mm
Column Length 250 mm
Particle Size 3 µm
Column Format Analytical Column
Packing Material Spherical Fully Porous Base-Deactivated Silica
End-Capped Yes
USP Classification L1
Pore Size 130 Å
Carbon Load 11%
Surface Area 170 m²/g
pH Range 2–9
Maximum Pressure 400 bar (5800 psi)
Maximum Temperature 60°C
Recommended Technique Reversed-Phase HPLC
Typical Applications Pharmaceutical, Chemical, Food, Environmental & Biotechnology Analysis

Features

  • Premium reversed-phase analytical HPLC column
  • Thermo Scientific™ Hypersil™ BDS product line
  • Column dimensions: 250 × 4.6 mm
  • 3 µm spherical fully porous silica particles
  • Base-Deactivated Silica (BDS) technology
  • C18 bonded stationary phase
  • End-capped stationary phase
  • Minimal residual silanol activity
  • Excellent peak symmetry for basic compounds
  • High chromatographic efficiency
  • Exceptional batch-to-batch reproducibility
  • USP L1 classification
  • 130 Å pore size
  • 11% carbon loading
  • Surface area of 170 m²/g
  • Operating pH range: 2–9
  • Maximum operating pressure: 400 bar (5800 psi)
  • Maximum operating temperature: 60°C
  • Suitable for routine HPLC analysis and QA/QC laboratories

Benefits

  • Excellent Peak Symmetry: Base-deactivated silica technology minimizes silanol interactions, improving peak shape for basic and polar analytes.
  • High Separation Efficiency: The 3 µm particle size provides excellent resolution, faster separations, and improved chromatographic performance.
  • Outstanding Reproducibility: Manufactured using Hypersil silica technology to ensure consistent performance between columns and analytical runs.
  • Wide Application Range: Suitable for pharmaceutical analysis, food testing, environmental monitoring, chemical analysis, and biotechnology research.
  • Extended Column Lifetime: Robust silica packing and end-capped C18 chemistry provide excellent durability under routine laboratory conditions.
  • Reliable Method Transfer: USP L1 classification makes the column suitable for validated HPLC methods and quality control laboratories.
  • Broad pH Compatibility: Operates reliably across a pH range of 2–9 for diverse analytical applications.
  • Reduced Secondary Interactions: Minimal residual silanol activity improves reproducibility and reduces analyte tailing.

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