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Hypersil ODS 250 x4.6mm 5 micron

The Thermo Scientific™ Hypersil™ ODS C18 HPLC Column (250 × 4.6 mm, 5 µm) is a high-performance reversed-phase analytical HPLC column designed for the reliable separation of acidic, neutral, moderately polar, and non-polar compounds. Built on high-purity Type A spherical silica with a bonded C18 (ODS) stationary phase, this column delivers exceptional reproducibility, high efficiency, and consistent chromatographic performance for routine analytical and quality control laboratories.

The Hypersil™ ODS column is recognized worldwide as an industry-standard HPLC column and is referenced in numerous validated analytical methods across pharmaceutical, environmental, food, biotechnology, and chemical laboratories. Its 250 mm length, 4.6 mm internal diameter, and 5 µm particle size provide excellent resolution and peak separation for complex sample matrices.

SKU: Model 30105-254630 Category: Brand:

Specifications

Parameter Specification
Product Type Reversed-Phase HPLC Column
Brand Thermo Scientific™
Product Line Hypersil™ ODS
Stationary Phase C18 (ODS)
USP Classification L1
Column Dimensions 250 × 4.6 mm
Particle Size 5 µm
Pore Size 120 Å
Surface Area 170 m²/g
Carbon Load 10%
Packing Material Spherical, Fully Porous Type A Silica
Column Type Analytical Column
Endcapped Yes
pH Range 2–8
Maximum Pressure 400 bar (5800 psi)
Maximum Temperature 60°C
Application Reversed-Phase HPLC Analysis

Features

  • Thermo Scientific™ Hypersil™ ODS C18 HPLC analytical column
  • Column dimensions: 250 × 4.6 mm
  • Particle size: 5 µm
  • General-purpose reversed-phase C18 stationary phase
  • High-purity Type A spherical silica packing
  • Endcapped stationary phase for improved peak performance
  • Suitable for acidic and neutral analytes
  • Excellent chromatographic reproducibility
  • High column efficiency and resolution
  • 120 Å pore size
  • Surface area: 170 m²/g
  • Carbon loading: 10%
  • pH operating range: 2–8
  • Maximum operating pressure: 400 bar (5800 psi)
  • Maximum operating temperature: 60°C
  • USP Classification: L1
  • Manufactured under ISO 9001 quality standards
  • Suitable for pharmaceutical, food, environmental, and research laboratories

Benefits

  • Excellent Separation Performance: Delivers high-resolution separation of acidic, neutral, moderately polar, and hydrophobic compounds with excellent peak symmetry.
  • Industry-Proven Reliability: Hypersil™ ODS columns are widely referenced in validated HPLC methods, making them ideal for routine analytical and QC applications.
  • Consistent Reproducibility: Manufactured under ISO quality standards to ensure reliable batch-to-batch chromatographic performance.
  • Versatile Analytical Applications: Suitable for pharmaceutical analysis, environmental testing, food safety, biotechnology, and chemical analysis.
  • Optimized Chromatographic Efficiency: The 250 mm column length provides enhanced resolution for complex sample separations.
  • Stable Operating Conditions: Compatible with mobile phases across a pH range of 2–8 and operating pressures up to 400 bar.
  • Long Column Lifetime: High-quality bonded C18 stationary phase and durable silica support provide dependable long-term performance.
  • Compatible with Standard HPLC Systems: The analytical column format (250 × 4.6 mm) is compatible with most conventional HPLC instruments.

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