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Hypersil ODS (C-18) 250 mm x 3.0 mm ID x 5 um

The Thermo Scientific™ Hypersil™ ODS (C18) HPLC Column (250 mm × 3.0 mm ID × 5 µm) is a high-performance reversed-phase analytical column designed for reliable and reproducible chromatographic separations. Built with fully porous Type A silica and an endcapped C18 stationary phase, this column is ideal for the analysis of acidic, neutral, and moderately non-polar compounds across pharmaceutical, environmental, food, biotechnology, and research laboratories.

Recognized as an industry-standard HPLC column, the Hypersil™ ODS series delivers excellent peak symmetry, high separation efficiency, and consistent reproducibility. The 250 mm column length provides enhanced resolution for complex sample separations, while the 3.0 mm internal diameter helps reduce solvent consumption without compromising chromatographic performance.

SKU: 30105-253030 Category: Brand:

Specifications

Parameter Specification
Product Type HPLC Analytical Column
Brand Thermo Scientific™
Product Line Hypersil™ ODS
Stationary Phase C18 (ODS)
Column Type Reversed Phase
Column Length 250 mm
Internal Diameter 3.0 mm
Particle Size 5 µm
Packing Material Fully Porous Type A Silica
Pore Size 120 Å
Carbon Load 10%
Surface Area 170 m²/g
USP Classification L1
Endcapped Yes
Operating pH Range 2–8
Maximum Operating Pressure 400 bar (5800 psi)
Maximum Temperature 60°C
Application General Purpose HPLC Analysis

Features

  • Thermo Scientific™ Hypersil™ ODS (C18) HPLC Column
  • Column dimensions: 250 mm × 3.0 mm ID
  • 5 µm particle size
  • Reversed-phase C18 stationary phase
  • Endcapped silica for improved peak symmetry
  • Fully porous Type A silica packing
  • General-purpose analytical HPLC column
  • Suitable for acidic and neutral analytes
  • High chromatographic efficiency
  • Excellent batch-to-batch reproducibility
  • 120 Å pore size
  • 10% carbon loading
  • pH operating range: 2–8
  • Maximum operating pressure: 400 bar (5800 psi)
  • Compatible with standard HPLC systems
  • Manufactured under ISO quality standards

Benefits

  • Excellent Separation Performance: Provides high-resolution separation of acidic, neutral, and moderately non-polar compounds.
  • Reliable Reproducibility: Delivers consistent chromatographic results, making it suitable for routine analytical and quality control laboratories.
  • Reduced Solvent Consumption: The 3.0 mm internal diameter helps lower mobile phase usage while maintaining analytical performance.
  • High Column Efficiency: The 5 µm particle size ensures sharp peaks, improved sensitivity, and reliable quantitative analysis.
  • Versatile Analytical Applications: Suitable for pharmaceutical analysis, environmental testing, food analysis, biotechnology, and chemical research.
  • Stable Reversed-Phase Chemistry: Endcapped C18 stationary phase minimizes secondary interactions and improves peak shape.
  • Long Service Life: High-quality silica packing provides durability and dependable performance under routine laboratory conditions.
  • Broad Method Compatibility: Compatible with a wide range of HPLC methods and commonly used mobile phases.

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